EFFECTS OF SELECTION IN TERMS OF MEAT YIELD TRAITS ON LEPTIN RECEPTOR GENE IN JAPANESE QUAIL LINES

Abstract: This study was carried out to investigate the effects of selection on single nucleotide polymorphisms (SNPs) in coding sequence leptin receptor (LEPR) gene and possible associations between SNPs’ some meat yield traits Japanese quail lines. Fifteen generations divergently selected two lines (HBW LBW) for 5-weeks age body weight a control were used as materials this study. A 348-bp part LEPR region (18th exon) sequenced total 113 individuals from three shown that fragments contained four SNPs loci (T490C, C528T, G537A, T571C) five haplotypes (TTGT, CTGT, TCGT, TCAT, TCAC). T490C replacement caused missense mutation phenylalanine convert leucine (PheLeu). However, other synonymous there no changes transcripts. It determined quails with higher phenotypic values TT genotype at T390C locus. Statistical analyses showed significant differences among lines, SNP alleles terms interested (P0.05), also haplotype distributions changed depending (P0.001). When all results evaluated together, it concluded fifteen economically important (C. coturnix japonica). Key words: SNP; haplotype; receptor; selection; quail0.05) japonica).Key quailUČINKI SELEKCIJE LINIJ JAPONSKIH PREPELIC NA GEN ZA LEPTINSKI RECEPTOR, POVEZAN S PRIREJO MESAIzvleček: Ta študija je bila izvedena z namenom raziskati učinke selekcije na polimorfizme posameznih nukleotidov (Angl., Single Nucleotide Polymorphism, SNP) v kodnem zaporedju gena za leptinski možne povezave med nekaterimi značilnostmi prireje mesa pri japonskih prepelicah. V študiji bilo poleg kontrole uporabljenih petnajst generacij različno izbranih dveh linij s telesno maso starosti petih tednov. del kodne regije (18. ekson) bil sekvenciran skupno posameznikih iz treh prepelic, fragmenti pa so vsebovali štiri lokuse pet haplotipov Zamenjava povzročila drugačnosmiselno mutacijo fenilalanina levcin (Phe Leu), vendar bili drugi SNP-ji sinonimni transkriptih ni sprememb. Ugotovljeno bilo, da prepelice višjimi fenotipskimi vrednostmi imele genotip lokusu T390C. Statistične analize vidika fenotipskih lastnosti pokazale značilne razlike linijami aleli haplotipi (P 0,05), prepelic različna tudi porazdelitev 0,001). Na podlagi vrednotenja vseh rezultatov smo ugotovili, selekcija petnajstih japonica) ključne spremembe genu LEPR, povezanim gospodarsko pomembnimi lastnostmi prepelic.Ključne besede: haplotip; selekcija; japosnka prepelica